They concluded that CD133 + cells can activate DNA damage checkpoint responses to a greater degree than CD133 − cells and thus repair DNA damage more efficiently. Intriguingly, by using an inhibitor of the checkpoint kinases Chk1 and Chk2, they were able to radiosensitize the CD133 + cells.

Here we show that cancer stem cells contribute to glioma radioresistance through preferential activation of the DNA damage checkpoint response and an increase in DNA repair capacity. The fraction

now show that GSCs have co-opted a neurodevelopmental program to activate Rac1 to promote defining features of GSCs. Our previous study showed that increased activation of the DNA damage response is implicated in radioresistance of the glioma stem cells . To determine the mechanisms through which Notch promotes radioresistance of glioma stem cells, we first assessed whether the Notch pathway affected activation of the checkpoint kinases after radiation. Wang et al. investigate reciprocal signaling between glioma stem cells and their differentiated glioblastoma cell progeny. The authors demonstrate that differentiated tumor cells promote the glioblastoma hierarchy and tumor growth through a paracrine feedback loop of neurotrophin signaling in cooperation with stem cell-like tumor cells.

The emerging role of cancer stem cells in tumor Glioblastoma is an aggressive and heterogeneous tumor in which glioblastoma stem cells (GSCs) are at the apex of an entropic hierarchy and impart devastating therapy resistance. The high entropy of GSCs is driven by a permissive epigenetic landscape and a mutational landscape that revokes crucial cellular checkpoints. In response to DNA damage, normal cells activate the DNA damage response (DDR), utilizing a variety of DNA damage sensing and repair pathways (e.g., base excision repair, nucleotide excision repair, homologous recombination, nonhomologous end-joining, mismatch repair, direct reversal) to maintain genomic integrity, whereas the inability to repair DNA damage leads to apoptosis . 2017-10-09 · Glioma stem cells promote radioresistance by preferential activation of the DNA damage response.

Google Scholar | Crossref | Bao, S., et al.

Ionizing radiation represents the most effective therapy for glioblastoma (World Health Organization grade IV glioma), one of the most lethal human malignancies, but radiotherapy remains only palliative because of radioresistance. The mechanisms underlying tumour radioresistance have remained elusive. Here we show that cancer stem cells contribute to glioma radioresistance through preferential activation of the DNA damage checkpoint response and an increase in DNA repair capacity.

Intriguingly, by using an inhibitor of the checkpoint kinases Chk1 and Chk2, they were able to radiosensitize the CD133 + cells. Glioma stem cells promote radioresistance by preferential activation of the DNA damage response. Autores: Shideng Bao, Qing Shi, Yueling Hao, Roger E. McLendon, Darell D. Bigner, Qiulian Wu, Anita B. Hjelmeland, Jeremy N. Rich, Mark W. Dewhirst Add your e-mail address to receive free newsletters from SCIRP.

2017-10-09 · Glioma stem cells promote radioresistance by preferential activation of the DNA damage response. Nature 2006; 444 : 756–760. CAS Article Google Scholar

Our previous study showed that increased activation of the DNA damage response is implicated in radioresistance of the glioma stem cells .

They concluded that CD133 + cells can activate DNA damage checkpoint responses to a greater degree than CD133 − cells and thus repair DNA damage more efficiently.
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Potential therapies that modulate or target cancer stem cells are also reviewed.

However G0S2 regulates glioma cell DNA repair in response to IR. Emerging evidence suggests that activation of DNA repair is an important factor for glioma radiation resistance [4, 5].
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Mario Capecchi describes the challenges in developing the technique of gene targeting, which allows the manipulation of genes from tissue culture cells to

Liu G, Yuan X, Zeng Z, et al. Analysis of gene expression and chemoresistance of CD133+ cancer stem cells in glioblastoma. Mol Cancer 2006;5:67. It has been reported that cancer stem cells may contribute to glioma radioresistance through preferential activation of the DNA damage checkpoint response and an increase in DNA repair capacity.

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Analysen är baserad på stamcells differentiering reporter varvid uttrycket av den förbättrade GFP Glioma stem cells promote radioresistance by preferential activation of the DNA damage response. neuromuscular blocking agents promote astroglial differentiation and deplete glioblastoma stem cells.

Blakeley J, Grossman S. Glioblastoma remains the most common and devastating primary brain tumor despite maximal therapy with surgery, chemotherapy, and radiation. The glioma stem cell (GSC) subpopulation has been identified in glioblastoma and likely plays a key role in resistance of these tumors to conventional therapies as well as recurrent disease. Glioma stem cells promote radioresistance by preferential activation of the DNA damage response.

Here we show that cancer stem cells contribute to glioma radioresistance through preferential activation of the DNA damage checkpoint response and an increase in DNA repair capacity. The fraction of tumour cells expressing CD133 (Prominin-1), a marker for both neural stem cells and brain cancer stem cells, is enriched after radiation in gliomas.

The mechanisms underlying tumour radioresistance have remained elusive. Here we show that cancer stem cells contribute to glioma radioresistance through preferential activation of the DNA damage checkpoint response and an increase in DNA repair capacity. cancer stem cells contribute to glioma radioresistance through cycles of IR also contained greater percentages of CD1331 cells than preferential activation of the DNA damage checkpoint response parental populations (Supplementary Fig. S2). Thus, tumours sur- Glioma stem cells promote radioresistance by preferential activation of the DNA damage response Here we show that cancer stem cells contribute to glioma radioresistance through preferential activation of the DNA damage checkpoint response and an increase in DNA repair capacity. The fraction It has been reported that cancer stem cells may contribute to glioma radioresistance through preferential activation of the DNA damage checkpoint response and an increase in DNA repair capacity. We have examined DNA repair in five stem and nonstem glioma cell lines. The population doubling time was … Comparative analysis of DNA repair in stem and nonstem glioma cell cultures.

The population doubling time was … 2018-05-21 · Previous studies showed that, preferential activation of the DNA damage checkpoint and enhanced DNA repair capacity in gliomas lead to radioresistance [9, 14, 15]. Strategies depending on targeting DNA damage response network in gliomas were applied to sensitize tumors and reverse radioresistance [16, 17]. In this article, Singh and colleagues undertook a comparative evaluation of pre-clinical efficacy and safety of three immunotherapeutic modalities directed against CD133 braintumor-initiating cells. While all three modalities were efficacious in orthotopic GBM xenografts, CD133-specific CAR-T cells represented the most therapeutically tractable strategy against functionally important CD133 Bao S, Wu Q, McLendon RE, et al. Glioma stem cells promote radioresistance by preferential activation of the DNA damage response. Nature. 2006; 444 (7120):756–760.